Journal: iScience

Article Title: Macrophage ferroportin serves as a therapeutic target against bacteria-induced acute lung injury by promoting barrier restoration

doi: 10.1016/j.isci.2022.105698

Figure Lengend Snippet:

Article Snippet: The following antibodies were used in the experiments: FITC Mouse Anti-Human CD45 (BD Pharmingen, 555482), eBioscience™ Fixable Viability Dye eFluor™ 455UV (ThermoFisher, 65-0868-14), BV510 Mouse Anti-Human CD11b (BD Pharmingen, 563088), Alexa Fluor® 700 Mouse Anti-Human HLA-DR (BD Pharmingen, 560743), PerCP-Cy™5.5 Mouse anti-human CD15 (BD Pharmingen, 560828), human ferroportin/SLC40A1 antibody (R&D, MAB9924) and Alexa Fluor 594-labeled donkey anti-rabbit secondary antibody (ThermoFisher, A-21207) were used.

Techniques: Blocking Assay, Diagnostic Assay, Recombinant, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Software, Expressing

Journal: iScience

Article Title: CIITA promoter polymorphism impairs monocytes HLA-DR expression in patients with septic shock

doi: 10.1016/j.isci.2022.105291

Figure Lengend Snippet: Monocyte HLA-DR expression in 203 patients with septic shock Circulating monocyte HLA-DR expression is expressed as number of antibodies bound per cell (AB/C). Results are presented as median with 95% CI and by genotype of CIITA rs12596540 and G-286A∗rs3087456 in a dominant model. ∗p< 0.03 vs. basal (Wilcoxon signed rank test).

Article Snippet: PE-Cy™7 Mouse Anti-Human HLA-DR , BD Biosciences , Cat#560651; RRID: AB_1727528.

Techniques: Expressing

Journal: iScience

Article Title: CIITA promoter polymorphism impairs monocytes HLA-DR expression in patients with septic shock

doi: 10.1016/j.isci.2022.105291

Figure Lengend Snippet: Ex vivo HLA-DR expression in monocytes of healthy controls Results are presented as violin plot with median and quartiles lines, and by genotype of CIITA G-712A∗rs12596540 and G-286A∗rs3087456 in an A-dominant model (results for the co-dominant model presented in Figure S2 ). p values were calculated using Mann-Whitney Test. ∗∗∗∗p< 0.0001 and ∗p< 0.05.

Article Snippet: PE-Cy™7 Mouse Anti-Human HLA-DR , BD Biosciences , Cat#560651; RRID: AB_1727528.

Techniques: Ex Vivo, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: CIITA promoter polymorphism impairs monocytes HLA-DR expression in patients with septic shock

doi: 10.1016/j.isci.2022.105291

Figure Lengend Snippet:

Article Snippet: PE-Cy™7 Mouse Anti-Human HLA-DR , BD Biosciences , Cat#560651; RRID: AB_1727528.

Techniques: Fluorescence, Quantitation Assay, Binding Assay, Recombinant, Luciferase, Transfection, Software, Plasmid Preparation, Mutagenesis